अहमदाबाद प्लेन क्रैश के बाद चर्चा में आया 'DNA टेस्ट', जानिए कैसे और कितने समय में होता है, यहां समझें पूरा प्रॉसेस

ETVBHARAT

डीएनए टेस्ट को लेकर ईटीवी भारत ने सर गंगाराम अस्पताल के जेनेटिक्स एवं जीनोमिक्स डिपार्टमेंट की वाइस चेयरमैन डॉ. सुनीता बिजारनिआ से बातचीत की.

Transcript

00:00After 241 people who have died, the dead body is not aware of it.

00:10So, the government has a DNA test compulsory.

00:13We are the vice chairman of the Genetics and Genomics Department.

00:20We will know what the DNA test is and how the dead body matches it.

00:29So, the DNA is basically an essence of our life.

00:36The DNA is passed on generation to generation.

00:39So, the DNA is unique to each human.

00:44It is a unique code.

00:47So, the DNA is a very good way to understand the DNA.

00:53Basically, it will need its relatives.

00:57Because the DNA sequence or the fingerprint,

01:03it needs to match someone's needs.

01:07So, we have to prove that this donor is a mother or a papa.

01:19So, we test the relationship to the DNA.

01:24So, I think the government has a step.

01:27This is correct.

01:28Because it will identify with the DNA.

01:31This particular person is a mother.

01:34This is for their identity.

01:38So, how can we do this process?

01:40How can we do this process?

01:41How can we do this process?

01:42And how can we do this process?

01:43Yes.

01:44So, in the lab, there is a routine process.

01:47There is a DNA that needs to be.

01:49Now, DNA is in our body of a cell.

01:53Except red blood cells.

01:55Of course.

01:56Of course.

01:57But, in this situation, there is no blood.

02:01But, any body can be taken away from it.

02:04And it can be extracted from it.

02:06It can be taken away from it.

02:07It can be taken away from it.

02:08It can be taken away from it.

02:09It can be taken away from it.

02:10If any body can be taken away from it.

02:12If the DNA is removed from it,

02:14then the relatives will take blood from it.

02:17And the blood will extract DNA from them.

02:20And the three will be checked simultaneously.

02:23And the parts will be checked.

02:26And they will try to match it.

02:29If some DNA later comes from it,

02:31then it will get 100% matching.

02:35When we have two parents.

02:38So, how does this testing happen?

02:40Do we see it?

02:41Do we see it?

02:42Do we see it?

02:43Do we see it?

02:44Do we see it?

02:45In our lab,

02:46we have a fingerprinting DNA.

02:48Because the first fingerprint was used.

02:51Just to get the identity of the person.

02:54Now, we do it with DNA.

02:56So, every DNA has some unique things.

03:01The DNA is a sequence.

03:05And, in there are some segments.

03:08We use satellites.

03:11Like, there are six letters of a sequence.

03:13That is one sequence that would be repeated twice,

03:15Normally.

03:18It has happened.

03:19In some individuals, it will repeat it twice.

03:22In some individuals,

03:23it will repeat it twice.

03:25And in some individuals, it will repeat it twice.

03:29My mother and my father.

03:31So these satellites, we recognize them, saying that satellite number 23 has 1 slash 2 repeats.

03:39After that, we check the parents and see how many of them are.

03:43They have 2 slash 3.

03:45The other parent has 1 slash 4.

03:48So, where has it come from?

03:51It comes to us.

03:53Similarly, we don't only check one thing.

03:56At least, 10 to 20 points, which are polymorphic.

04:04In that particular region, there are a lot of variations.

04:09Each individual, there is no difference between each individual.

04:13So these regions are checked from DNA.

04:17This is a DNA-based test.

04:19There are many ways in the laboratory.

04:22But it is a streamlined process.

04:25So, if we cut these three, then it will be more useful.

04:28How much do we do in the environment?

04:30In the environment?

04:31Yes.

04:32Well, if we use the DNA extraction,

04:34if we use it from the kit,

04:35then it will get out of 15-20 minutes.

04:38But if we cut the DNA good,

04:40then it will take one day.

04:42So, if we take 10 tests,

04:44if we take 10 tests,

04:45then we can take 10 tests together.

04:47Then we can take 10 tests together,

04:48and then we can take 10 tests together.

04:50So, minimum to minimum,

04:52I think,

04:53it will take 2 or 3 days.

04:55And average,

04:56it will take 1 week.

04:57And then,

04:58if there is a human being,

05:00then we have to do it.

05:01And if there is a human being,

05:02then there is a difference in the process?

05:04No,

05:05the process is the same.

05:06The process is the same.

05:07The process is the same.

05:08Where is the DNA taken from?

05:09So,

05:10the living human being,

05:11we don't take blood.

05:12That is the most conventional tissue

05:14to take for DNA extraction.

05:16But,

05:17the human being,

05:18whatever the disease is needed,

05:19can be used.

05:21So,

05:22if anyone has gone through,

05:23then the bone is only available.

05:25So,

05:26that could be done with that?

05:27Yes,

05:28if you have bone,

05:29it will be perfect.

05:30Yes,

05:31it remains the same.

05:32Or,

05:33blood,

05:34bone,

05:36bone,

05:37liver,

05:38such as

05:39If someone's body is gone,

05:41it is only the same.

05:42So,

05:43how many years after this

05:44can happen,

05:45So, DNA is more stable but over the years it definitely degrade.

05:52So, if we take blood, if we put DNA in the room, then it will be fine for 2-4 days.

05:58Then it will degrade over months.

06:01So, quality will not be so good.

06:04But it is possible to identify a person using the DNA.

06:09So, DNA is correct, there is no variation or difference in it?

06:16No, there is no difference in it.

06:19But what happens is that every person is different.

06:22And as we have a new PD, there are new changes in each PD.

06:27So, sometimes we get a change.

06:30As we expect that we have two letters in one point.

06:35One should come from a mother and one should come from a father.

06:38Sometimes it doesn't match each letter.

06:41It doesn't match each letter.

06:42But the mother doesn't match each letter.

06:44So, it doesn't mean that she doesn't have a mother.

06:46It means that it has a new variation.

06:48The ability changes.

06:49Thank you very much.

Category

Transcript

Recommended