CCK-8 Assay: A Reliable and Convenient Cell Viability Method for Biological Research | GlpBio

  • 11 months ago
CCK-8, often known as the Cell Counting Kit-8, is a frequently used cell viability assay in biological research. It is a quick and easy way to count the number of viable cells in a culture.

The CCK-8 assay relies on dehydrogenases in living cells to convert a tetrazolium salt, WST-8, to a coloured formazan product. The amount of formazan produced in the culture is directly related to the number of viable cells.

CCK8 offers the ultimate solution for cell viability analysis, providing researchers with a powerful and reliable method to assess the viability and proliferation of cells with exceptional accuracy.

To perform the CCK-8 assay, a tiny amount of the reagent is applied to a cell culture and cultured for a brief time. The absorbance of the resultant solution is then measured at 450 nm using a microplate reader. To determine the amount of live cells in the culture, the absorbance is compared to a standard curve.

The CCK-8 assay is very sensitive and can detect minor changes in cell viability, making it an invaluable tool for drug discovery, toxicity testing, and cell proliferation investigations. It is also reasonably priced and simple to use, making it a popular choice among scholars.

Get more information form here: https://www.glpbio.com/cell-counting-kit-8-cck-8.html

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